Genetic Diversity Of Cladosporium Species Isolated From Air Of Cairo, Egypt Using Microsatellite Primed Pcr (Mp-pcr) And Universally Primed Pcr (Up-pcr) Techniques

Mohammed Salah Abdulrahman, TEACHING ASSISTANT OF MICROBIOLOGY AND IMMUNOLOGYFollow
Hamido M. Hefny
Moselhy S. Mansy
Amal A. I. Mekawey

Subject Area

Microbiology and Immunology

Abstract

The samples were then identified by macroscopic and microscopical methods, Molecular fingerprinting, of Cladosporium strains populations depending on MP-PCR using Primers (AGG) 5 , (CAG) 5 , (GACA) 4 , and (AG) 8 C. In addition, we have applied the UP-PCR technique using L21 and Fok1 Genotyping patterns extracted by MP-PCR showed good discrimination with amplification of (AGG) 5 , (AG) 8 C and (CAG) 5 over (GACA) 4. In addition, L21 primer revealed variability over FOK1 primer. current work indicate that MP-PCR and UP-PCR fingerprinting method are appropriate for characterization of large groups of Cladosporium due to its simplicity

Recommended Citation

Abdulrahman, Mohammed Salah; Hefny, Hamido M.; Mansy, Moselhy S.; and Mekawey, Amal A. I. (2022) "Genetic Diversity Of Cladosporium Species Isolated From Air Of Cairo, Egypt Using Microsatellite Primed Pcr (Mp-pcr) And Universally Primed Pcr (Up-pcr) Techniques," Bulletin of Faculty of Pharmacy Cairo University: Vol. 60 : Iss. 1 , Article 2.
Available at: https://doi.org/10.54634/2090-9101.1028