GC and HPTLC- Densitometric methods for Simultaneous Determination of Aspirin, Paracetamol, Caffeine Anhydrous, Dextromethorphan Hydrobromide and Chlorpheniramine Maleate: Method validation and application to Over the Counter Tablet

Authors

Lamia M. Abdel-Halim, Central Administration for Drug Control, Egyptian Drug Authority, Wezaret Al Zeraa Street, 12311, Giza, Egypt
Nesrin K. Ramadan, Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562, Cairo, Egypt
Mohamed K. Abd-El Rahman, Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562, Cairo, Egypt
Maha M. Galal, Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562, Cairo, EgyptFollow

Subject Area

General Chemistry

Abstract

This study presents two validated chromatographic methods for determining aspirin, paracetamol, caffeine anhydrous, dextromethorphan hydrobromide, and chlorpheniramine maleate in Vegaskin-D® tablets. Method I used high performance thin layer liquid chromatography densitometric method (HPTLC-densitometric), using a stationary phase of silica gel 60 F254, and UV detection at 254.0 nm resulting in well-resolved, symmetrical, and sharp peaks. Method II used gas chromatography (GC) with a flame ionization detector. The results showed that the proposed methods can effectively be implemented for routine analysis and quality control of drugs in fixed-dose combination tablets. The GC method has an advantage over HPTLC- densitometric method due to the separation of salicylic acid; main degradation product of Aspirin

Recommended Citation

Abdel-Halim, Lamia M.; Ramadan, Nesrin K.; Rahman, Mohamed K. Abd-El; and Galal, Maha M. (2023) "GC and HPTLC- Densitometric methods for Simultaneous Determination of Aspirin, Paracetamol, Caffeine Anhydrous, Dextromethorphan Hydrobromide and Chlorpheniramine Maleate: Method validation and application to Over the Counter Tablet," Bulletin of Faculty of Pharmacy Cairo University: Vol. 61 : Iss. 1 , Article 7.
Available at: https://doi.org/10.54634/2090-9101.1043

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